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Protein detection

 

Pfu DNA polymerase

Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75¢XC, catalyzing the polymerisation of nucleotides into duplex DNA in the 5¡¦¡X> 3¡¦direction. Pfu DNA Polymerase possesses 3¡¦ ¡X> 5¡¦ exonuclease (proofreading) activity. Nucleotide misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity synthesis. Pfu DNA Polymerase generated PCR fragments are blunt ¡V ended.

Product list

Concentration: 5000 units/ml

Reaction buffer (10x): Ammonium buffer with 20 mM MgSO 4

Storage buffer: 50 mM TrisHCl (pH 8,2), 0.1 mM EDTA, 1mM DTT, 0,1% Nonidet P40, 0,1% Tween-20, 50% glycerol.

Storage conditions: -20¢XC

Ordering informatiom

Cat No
Description
Pack size
Certificate
Price $
PF0500
Pfu DNA polymerase
500U
PF2500
Pfu DNA polymerase
2500U

 

 

 

 

 

ZymesetBiotek
email: max@zymeset.com

 

 

 

 

Pfu DNA polymerase activity test